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以多聚酶链反应技术扩增获得了杜氏利什曼原虫（Ｌ．ｄｏｎｏｖａｎｉ）热休克蛋白７０（ＨＳＰ７０）全长基因序列，构建ＤＮＡ疫苗表达载体ｐＶＲ１０２０－ＨＳＰ７０。以脂质体法体外转染小鼠成纤维细胞系ＳＶＴ２，应用Ｗｅｓｔｅｒｎｂｌｏｔ法证实构建的表达载体具有表达ＨＳＰ７０的能力。以１０μｇ的ＤＮＡ疫苗表达载体质粒经肌肉、皮下注射进行免疫，２周后即可出现抗－ＨＳＰ７０的抗体应答。建立了ＤＮＡ疫苗免疫应答的模型。
The full-length heat shock protein 70 (HSP70) gene sequence of L. donovani was amplified by polymerase chain reaction and the DNA vaccine expression vector pVR1020-HSP70 was constructed. The mouse fibroblast cell line SVT2 was transfected by liposome in vitro. Western blot was used to confirm the expression of HSP70. Immunization with a 10 μg DNA vaccine expression vector plasmid followed by subcutaneous injections resulted in an anti-HSP70 antibody response two weeks later. Established a model of DNA vaccine immune response.