论文部分内容阅读
天龙通心方(TLTX)是由红景天、丹参、川芎等6味中药组方而成,临床主要用于气虚血瘀所致的胸痹。为探讨TLTX药效物质基础,并为给药方案提供依据,给予大鼠TLTX后对7种成分的血药浓度和药代特征进行了研究。建立了同时检测生物样品中7种化合物的高灵敏度LC-MS/MS分析方法,经过对线性、专属性、回收率、准确度、精密度和稳定性考察,所建立的分析方法符合临床前药代动力学研究要求。灌胃TLTX 4.5 g·kg~(-1)后在大鼠血浆中检出7种成分,并分别在给药后0.11~4.67 h达峰。各成分体内含量差异较大,其中红景天苷的药时曲线下面积明显高于其他成分,这表明该成分可能是复方中的主要起效成分,也从体内血药浓度角度说明了红景天为君药的科学性。
Tianlong Tongxinfang (TLTX) is composed of six herbs of Rhodiola, Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong, and is mainly used for chest paralysis caused by Qi-deficiency and blood stasis. In order to explore the material basis of TLTX pharmacodynamics and to provide a basis for drug administration, the blood concentration and pharmacokinetic characteristics of seven components were studied after TLTX administration in rats. A high-sensitivity LC-MS / MS method for the simultaneous determination of seven compounds in biological samples was developed. The analytical methods established for linearity, specificity, recovery, accuracy, precision and stability are in accordance with the preclinical drug A generation of dynamic research requirements. After inoculation of TLTX 4.5 g · kg -1, seven components were detected in the plasma of rats and peaked at 0.11 ~ 4.67 h after administration. The content of each component varies greatly, and the area under the curve of salidroside is significantly higher than other components, which indicates that the component may be the main active ingredient in the compound, and also illustrates the redness from the perspective of the blood concentration in vivo God is the science of medicine.