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以六个粳型杂交稻的幼穗为外植体 ,诱导愈伤组织并分化为胚性细胞 ,经秋水仙素处理 ,使胚性细胞染色体加倍为四倍体。共获得 2 43个四倍体株系 ,再由四倍体花药培养成双单倍体。观察到 ,这种四倍体性状稳定 ,但在少数株系中出现二倍体 ,此为无融合生殖的双单倍体。在获得的 86个双单倍体不同株系之间表现遗传的多样性 ,大多数株系性状稳定 ,少数株系有分离。经对性状的考查分析 ,双单倍体的株系中有少数株系产量优势接近或超过原供体亲本 ,且具有许多优良性状。可见由粳型杂交稻培养双单倍体也是改良品种的一种方法。
The young spikes of six japonica hybrid rice were used as explants, the callus was induced and differentiated into embryogenic cells. After colchicine treatment, the chromosomes of embryogenic cells were doubled to tetraploids. A total of 2 43 tetraploid strains were obtained, which were then double haploid cultivated by tetraploid anther. This tetraploid trait was observed to be stable, but diploids appeared in a few strains, which are the double haploid for apomixis. Genetic diversity was observed among the 86 doubled haploid lines, most of the lines were stable and a few isolated. According to the analysis of the traits, the yield advantage of a few of the doubled haploid lines is close to or more than the original donor parent, and has many excellent traits. Can be seen from japonica hybrid rice double haploid is also a way to improve varieties.