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目的:分离马齿苋(Portulaca oleracea L.)水提取物中生物碱类化学成分。方法:采用水煎煮提取、乙酸乙酯萃取、硅胶柱色谱、ODS柱色谱、葡聚糖柱色谱等技术进行提取、分离和纯化,再用超高效液相色谱进行制备,利用高分辨质谱和核磁共振等技术进行结构鉴定。结果:HR-ESI-TOF-MS、~1H-NMR、~(13)C-NMR、DEPT和2D NMR的结果与有关文献对照,确定所得化合物为N-反式-阿魏酰酪胺和N-反式-阿魏酰-3-甲氧基酪胺。结论:该实验从马齿苋中分离得到两种酪胺类生物碱:N-反式-阿魏酰酪胺和N-反式-阿魏酰-3-甲氧基酪胺。
Objective: To separate the chemical constituents of alkaloids from the water extract of Portulaca oleracea L. Methods: Extraction, separation and purification were carried out by boiling water extraction, ethyl acetate extraction, silica gel column chromatography, ODS column chromatography and dextran column chromatography, and then were separated by ultra performance liquid chromatography and identified by high resolution mass spectrometry and NMR and other technologies for structural identification. Results: The results of HR-ESI-TOF-MS, ~ 1H-NMR, ~ (13) C-NMR, DEPT and 2D NMR were compared with those of the related literature to confirm that the obtained compound was N-trans-ferulicotylamine and N- Feruloyl-3-methoxytyrosamine. Conclusion: This experiment isolated two kinds of tyramide alkaloids from the purslane: N-trans-ferulic acid tyramide and N-trans-feruloyl-3-methoxy tyramine.