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目的分析PTEN在结肠癌中的表达及临床意义。方法采用免疫组织化学技术及RT-PCR技术检测65例结肠癌患者癌及癌旁组织PTEN表达情况,分析不同临床病理特征结肠癌患者PTEN表达类型。结果结肠癌患者中PTEN高表达型和PTEN半低表达型比例均显著高于PTEN低表达型(P<0.001);PTEN mRNA在PTEN高表达型和PTEN半低表达型中的表达均显著高于PTEN低表达型(P<0.001);PTEN mRNA在PTEN高表达型癌组织中的表达高于PTEN半低表达型(P<0.001);中-高分化、Ⅰ-Ⅱ期、无肌层浸润患者以PTEN高表达型为主,而低-未分化、Ⅲ-Ⅳ期、有肌层浸润患者以PTEN半低表达型为主。PTEN表达类型与患者临床病理特征有关。结论不同临床病理特征结肠癌患者PTEN表达类型各异,PTEN半低表达型结肠癌患者肿瘤恶性程度更高。
Objective To analyze the expression and clinical significance of PTEN in colon cancer. Methods Immunohistochemistry and RT-PCR were used to detect the expression of PTEN in 65 cases of colorectal cancer and its adjacent tissues. The types of PTEN were analyzed in different clinical and pathological features of colon cancer. Results The percentages of PTEN high expression and PTEN low expression were significantly higher in patients with colon cancer than in PTEN low expression (P <0.001). The expressions of PTEN mRNA in PTEN high expression and PTEN low expression were significantly higher than those in PTEN low expression PTEN low expression (P <0.001). The expression of PTEN mRNA in PTEN high expression was higher than that in PTEN low expression (P <0.001) PTEN high expression type, while low - undifferentiated, Ⅲ-Ⅳ stage, with myometrial invasion of PTEN semi-low expression type based. The expression of PTEN is related to the clinicopathological features of patients. Conclusion There are different types of PTEN expression in different clinical and pathological features of colon cancer patients, and the degree of malignancy of PTEN semi-low expression colon cancer patients is higher.