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据物理学家组织网12月12日(北京时间)报道,英国研究人员简化了基因组测序的标准流程,首次无需进行文库制备便完成了DNA(脱氧核糖核酸)单分子测序。新方法仅需很少量的DNA就能获得序列数据,用量低于1纳克(10亿分之一克),为常规测序方法的1/500到1/600。文库制备是指从测序前基因组样本中提取不同长度的DNA片段,这一过程不仅费力、费时,还会浪费DNA,而新技术极大地减少了DNA的损耗,并缩短测序时间。该研究论文的第一作者、英国威康信托基金会桑格研究所的保罗.库普兰说:“用这种方法对病毒和细菌的基因组测序后发现,即使在相对较低的水平,也能够确定所检测的是何种有机
According to the December 12 (Beijing time) report of the Physicists’ Organization, British researchers simplified the standard sequencing of genome sequencing and, for the first time, completed single-molecule sequencing of DNA without the need for library preparation. The new method uses only a small amount of DNA to obtain sequence data at less than 1 nanogram (1 billionth of a gram), which is 1/500 to 1/600 of the usual sequencing methods. Library preparation refers to the extraction of DNA fragments of different lengths from pre-sequencing genomic samples, a process that is not only laborious, time-consuming, but also wastes DNA, while the new technology greatly reduces the loss of DNA and shortens the sequencing time. The first author of the study, Paul Cuppland of the Sanger Institute of Wellcome Trust in the UK, said: ”Sequencing of the virus and bacteria genomes in this way was found to be even at relatively low levels It is possible to determine what kind of organic substance is detected