骨髓移植后骨髓或淋巴造血组织的重建,有力地说明在人的骨髓或在末梢血中,存在着多能造血干细胞。对骨髓增殖性疾病的研究也支持这一观点。多能造血干细胞具有广泛的自我更新能力和多向分化的能力,细胞培养和单克隆抗体是识别多能造血干细胞和研究其调节功能的两种方法。Johnson,Metcalf 首先培养出鼠的多能 Reconstruction of bone marrow or lymphoid hematopoietic tissue after bone marrow transplantation strongly suggests that there is pluripotent hematopoietic stem cells in human bone marrow or in peripheral blood. Research on myeloproliferative diseases also supports this view. Pluripotent hematopoietic stem cells have a wide range of self-renewal and multidrug differentiation capabilities. Cell culture and monoclonal antibodies are two ways to identify pluripotent hematopoietic stem cells and to study their regulatory function. Johnson, Metcalf first cultivated mouse pluripotent