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本文使用LM、CLSM、SEM、TEM、EDS-X显微分析方法对年轻正畸病人的前磨牙进行成分及断层研究。 牙釉质中的釉梭、釉丛及釉板中的有机成分可以通过常规的光镜确认。并可由CLSM进一步确认。经测量,大多数的釉梭长度为50μm,厚度为4—7μm,大多数局限在牙尖顶部,SEM观察显示单个突起及釉梭从牙本质伸展入牙釉质,釉梭穿插在釉柱中,显示了造牙本质细胞突起远端的分支和聚合。EDS-X分析显示釉梭、釉丛及釉板的钙、磷重量百分比低于釉柱,而釉梭的钙、磷重量百分比高于釉丛与釉板,但这也可能是周围牙釉质的污染所造成的。对牙本质一牙釉质交界处的TEM观察也提示单个突起及釉梭为早期釉质形成过程中,造牙本质细胞突起伸展人牙釉质并由牙釉质包裹所形成。
In this paper, LM and CLSM, SEM, TEM, EDS-X microscopic analysis of young orthodontic patients premolar composition and tomography. Enamel in the glaze shuttle, glaze and glaze glaze organic components can be confirmed by conventional light microscopy. It can be further confirmed by the CLSM. Most of the glaze spines were measured to be 50 μm in length and 4-7 μm in thickness, mostly confined to the top of the cusp. SEM observations showed that a single protrusion and glaze shuttle extended from dentin into the enamel, the glaze shuttle interspersed in the glaze, Dendritic cells show the distal branches and aggregates of protuberances. The EDS-X analysis showed that the glauber, glaze and glaze had lower calcium and phosphorus contents than the glaze, while the Glauber's spindle had higher calcium and phosphorus contents than glaze and glaze but this may also be the surrounding enamel Caused by pollution TEM observation of the dentin-enamel junction also suggests that a single process of protrusion and glaze shuttle was associated with early enamel formation. Protrusion of the dentin cells stretched human enamel and formed by enamel wrapping.