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目的:探讨SH2-Bβ在哮喘发病中的作用。方法:用卵蛋白致敏致喘的方法制备BALB/c小鼠哮喘模型。应用免疫组织化学方法和免疫印记法(Western blot)观察SH2-Bβ在哮喘小鼠下呼吸道及内脏感觉传入部位的表达及应用SH2-Bβ抗体后SH2-Bβ水平的变化,Metamoph图象分析系统对结果进行分析。结果:免疫组织化学结果显示哮喘小鼠肺、C7-T5节段脊神经节及对应的脊髓后角SH2-Bβ免疫阳性产物平均光密度值分别为0.806±0.023、0.766±0.018、0.547±0.014,明显高于对照组(0.243±0.018、0.131±0.011、0.215±0.029)(P<0.01)。而应用SH2-Bβ抗体组相应部位的平均光密度值分别为(0.252±0.015、0.158±0.012、0.251±0.024)明显低于哮喘组(P<0.01)。Western blot结果显示:哮喘组肺及C7-T5节段脊髓后角SH2-Bβ与β-actin MOD值的比(2.512±0.021,2.615±0.022)明显高于对照组(0.675±0.019,0.712±0.018)(P<0.01),而应用SH2-Bβ抗体组相应部位的比值(0.969±0.023,1.023±0.022)明显低于哮喘组(P<0.01)。结论:肺内、C7-T5脊神经节及对应脊髓后角神经元的SH2-Bβ参与哮喘的发病过程,anti-SH2-Bβ可下调SH2-Bβ的表达,减轻肺部炎症。
Objective: To investigate the role of SH2-Bβ in the pathogenesis of asthma. Methods: BALB / c mouse asthma model was prepared by ovalbumin sensitization and asthma. The expression of SH2-Bβ in sensory afferent sites of lower respiratory tract and visceral organs of asthmatic mice and the change of SH2-Bβ level after using SH2-Bβantibody were observed by immunohistochemistry and Western blot. Metamoph image analysis system Analyze the results. Results: Immunohistochemistry results showed that the average optical density of SH2-Bβ immunoreactive products in the lung, C7-T5 segments of spinal cord and the corresponding spinal cord posterior horn of asthmatic mice were 0.806 ± 0.023,0.766 ± 0.018,0.547 ± 0.014, respectively (0.243 ± 0.018,0.131 ± 0.011,0.215 ± 0.029) (P <0.01). The average optical density values of the corresponding sites of SH2-Bβ antibody group were (0.252 ± 0.015,0.158 ± 0.012,0.251 ± 0.024), respectively, significantly lower than those of the asthma group (P <0.01). Western blot results showed that the ratio of SH2-Bβ to β-actin MOD in lung and C7-T5 segments of spinal cord in asthma group was significantly higher than that of control group (2.512 ± 0.021, 2.615 ± 0.022) (0.675 ± 0.019, 0.712 ± 0.018 ) (P <0.01). However, the ratios of the corresponding sites in the SH2-Bβ antibody group (0.969 ± 0.023, 1.023 ± 0.022) were significantly lower than those in the asthma group (P <0.01). Conclusion: SH2-Bβ in lung, C7-T5 spinal ganglion and corresponding spinal neurons in the spinal cord are involved in the pathogenesis of asthma. Anti-SH2-Bβ can down-regulate SH2-Bβ expression and reduce lung inflammation.