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本文采用组织化学和高效液相色谱等方法,对棉花感染枯萎病后体内棉酚及苯丙氨酸解氨酶(PA L.E.C.4.3.1.5)的变化进行了研究,试图探讨它们在棉花抗病生理中的作用。发现棉株感染枯萎病后,体内棉酚含量增加,PAL活性提高,并在品种间存在着显著差异,抗病品种比感病品种增加幅度较大。本文还通过棉酚对PAL粗提液的活性测定,发现棉酚浓度在2ppm～200ppm内.对PAL的活性具有显著的促进效应,提出棉酚在棉花体内不单是次生物质,它可能参与或影响着棉花的某些代谢过程,是值得进一步研究的领域。本文还提出了利用幼苗中棉酚和PAL活,胜的变化进行抗病性鉴定的可能性。
In this paper, the changes of gossypol and phenylalanine ammonia-lyase (PA LEC4.3.1.5) in cotton after Fusarium wilt were studied by means of histochemistry and high performance liquid chromatography (HPLC) In the role. It was found that cotton plant infected with Fusarium wilt, the gossypol content increased, PAL activity increased, and there is a significant difference between the varieties, resistant varieties than the susceptible varieties increased marginally. In this paper, the activity of gossypol in crude extract of PAL was measured and it was found that the concentration of gossypol was within 2ppm ~ 200ppm, which had a significant promotion effect on the activity of PAL. It was suggested that gossypol is not only a secondary biomass in cotton, Affect some of the metabolic processes of cotton, is worth further study. In this paper, we also proposed the possibility of utilizing the changes of gossypol and PAL activity in seedlings for the identification of disease resistance.