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目的:探讨沙眼衣原体(Ct)感染与分泌性中耳炎(SOM)发病的关系。方法:PCR检测157例(183耳)SOM患者的中耳积液(MEE),用荧光定量PCR(FQ-PCR)对阳性标本进行定量检测,用McCoy细胞对其中含高拷贝数基因的标本进行Ct分离培养。结果:PCR检测的183份MEE标本中,29份为阳性,FQ-PCR检测这些阳性标本基因拷贝数在2.0×106/L~6.0×1010/L,11耳高拷贝数基因的标本分离到1株Ct。结论:Ct感染与SOM发病有关,可能是导致SOM的致病微生物之一。
Objective: To investigate the relationship between Chlamydia trachomatis (Ct) infection and the pathogenesis of secretory otitis media (SOM). Methods: The middle ear effusion (MEE) of 157 patients (183 ears) with SOM was detected by PCR. The positive samples were quantitatively detected by fluorescence quantitative PCR (FQ-PCR). The samples with high copy number gene were detected by McCoy cells Ct culture. RESULTS: Of the 183 MEE specimens detected by PCR, 29 were positive. The positive copy number was 2.0 × 106 / L to 6.0 × 1010 / L in FQ-PCR. Strain Ct. Conclusion: Ct infection is related to the pathogenesis of SOM and may be one of the pathogenic microorganisms that cause SOM.