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将刀豆蛋白A(Con A)经尾静脉注射入雄性Balb/C小鼠,建立T细胞介导的Con A急性肝损伤小鼠模型,检测模型小鼠不同时间点血清中高迁移率族蛋白1(HMGB1)及肝组织HMGB1mRNA的表达水平,并与血清中TNF-α进行对比.实验显示:在Con A注射后0～3h,小鼠血清中的未能检测出HMGB1蛋白特异带;但在4h后,检测出一条微弱的HMGB1蛋白特异带;在6～36h,均检测出一条清晰的HMGB1蛋白特异带,在8￣12h处于一个高水平状态.而正常对照组小鼠血清中未能检测HMGB1蛋白.与HMGB1动力学曲线不同,血清中TNF-α水平在Con A注射后2h就达到峰值,4h后迅速下降,8h恢复到正常范围.Con A小鼠肝细胞中HMGB1mRNA的表达在Con A注射1h后即达到峰值,是对照组的2.4倍,之后迅速回落,3～12h均低于对照组水平(0.5～0.8倍),24h后逐渐恢复正常水平.初步揭示HMGB1在Con A小鼠急性肝损伤中可能发挥重要作用,血清中HMGB1蛋白与TNF-α相比,呈现出迟发与长效的特点,HMGB1给肝细胞造成损伤有足够强度和效应时间.
Con A was injected into male Balb / C mice via caudal vein to establish T cell-mediated mouse model of acute liver injury induced by Con A. The serum levels of high mobility group box 1 (HMGB1) and liver tissue HMGB1mRNA, and compared with serum TNF-α.Experimental results showed that HMGB1 protein could not be detected in serum of mice at 0 ~ 3h after Con A injection, but at 4h , A weak HMGB1 protein band was detected.A clear HMGB1 protein band was detected at 6 ~ 36h, which was at a high level at 8 ~ 12h.However, in the normal control group, HMGB1 Protein.Compared with the HMGB1 kinetic curve, the level of TNF-α in serum reached a peak at 2h after Con A injection, rapidly decreased after 4h and returned to the normal range at 8h.The expression of HMGB1mRNA in Con A mouse hepatocytes was significantly higher than that of Con A 1h, reached the peak 2.4 times that of the control group, then dropped rapidly, and then decreased rapidly from 3 to 12 hours (0.5 to 0.8 times), gradually returned to normal levels after 24 hours. Injury may play an important role in the serum HMGB1 protein compared with TNF-α, presented Delayed onset and long-term characteristics, HMGB1 damage to the liver cells have sufficient strength and effect of time.