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目的:了解职业苯接触及慢性苯中毒工人外周血中T细胞受体信号传导通路FcεRIγ基因的表达情况。方法:利用实时荧光定量PCR法分别检测22例职业苯接触工人、12例慢性苯中毒工人及9例正常人外周血单个核细胞(PBMC)中FcεRIγ基因表达情况。结果:正常人、职业苯接触及慢性苯中毒工人FcεRIγ基因表达水平的中位数分别为37.50%、12.65%、7.41%,职业苯接触及慢性苯中毒工人FcεRIγ基因表达与正常成人相比均显著下降(P<0.05);尽管慢性苯中毒工人外周血中FcεRIγ基因表达水平降低程度较职业苯接触更为明显,但二者之间无统计学意义(P>0.05)。结论:职业苯接触及慢性苯中毒工人外周血中T细胞FcεRIγ基因表达水平均下调,这种表达异常可能与接触苯后机体细胞免疫功能障碍有关。
OBJECTIVE: To understand the expression of FcεRIγ gene in peripheral blood of workers exposed to benzene and chronic benzene poisoning. Methods: The expression of FcsRIγ gene in peripheral blood mononuclear cells (PBMCs) from 22 workers exposed to benzene, 12 workers with chronic benzene poisoning and 9 normal controls were detected by real-time fluorescence quantitative PCR. Results: The median of FcεRIγ gene expression was 37.50%, 12.65%, 7.41% respectively in normal benzene, benzene and chronic benzene poisoning workers, and the expression of FcεRIγ in workers exposed to benzene and chronic benzene poisoning was significantly higher than that in normal adults (P <0.05). Although the expression of FcεRIγ in peripheral blood of chronic benzene poisoning workers was more obvious than that of professional benzene, there was no significant difference between them (P> 0.05). CONCLUSION: The levels of T cell FcεRIγ gene in peripheral blood of workers exposed to benzene and chronic benzene poisoning are both down-regulated, which may be related to the cellular immune dysfunction after exposure to benzene.