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采用ＫｒｏｍａｓｉｌＣ１８柱（２５０ｍｍ×４．６ｍｍ），流动相为磷酸二氢钾缓冲液（０．２ｍｏｌ／Ｌ）－甲醇－乙腈－水（４７３５３１５），检测波长２６５ｎｍ，头孢克洛呈现单一主峰，日内ＲＳＤ０．８２％。本方法快速、准确，与微生物检定方法结果接近。
The mobile phase consisted of potassium dihydrogen phosphate buffer (0.2mol / L) -methanol-acetonitrile-water (4735315) at a detection wavelength of 265nm with single peak of cefaclor and intra-day RSD0 using Kromasil C18 column (250mm × 4.6mm) .82%. The method is rapid and accurate, and the result of the method is close to that of the microorganism.