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目的研究沙利度胺(Tha)对SMMC7721移植瘤生长以及瘤组织Ki67和增殖性细胞核抗原(PCNA)表达的影响。方法 40只BALB/c裸鼠接种SMMC7721细胞,随机均分为分别在早期干预和正常干预条件下的Tha 25mg/kg处理组(A1和A2组)、Tha 2.5mg/kg处理组(B1和B2组)、环磷酰胺20mg/kg阳性对照组(C1和C2组)、生理盐水20ml/kg阴性对照组(D1和D2组)。观察移植瘤体积变化,免疫组化法检测瘤组织Ki67和PCNA的表达。结果与阴性对照组比较,Tha干预组、阳性对照组瘤体积以及瘤组织中Ki67和PCNA表达均明显减少(P<0.05)。此外,A1组瘤体积和Ki67、PCNA表达少于A2、B1组,A2、B1组少于B2组(P<0.05)。结论 Tha能抑制SMMC7721移植瘤生长,且早期、高剂量给药抑瘤效果更显著;其抑瘤机制与下调Ki67、PCNA蛋白表达进而抑制瘤细胞增殖相关。
Objective To investigate the effects of thalidomide (Tha) on the growth of SMMC7721 xenografts and the expression of Ki67 and proliferating cell nuclear antigen (PCNA) in tumor tissues. Methods 40 BALB / c nude mice were inoculated with SMMC7721 cells and randomly divided into Tha 25mg / kg group (A1 and A2), Tha 2.5mg / kg group (B1 and B2) under the condition of early intervention and normal intervention respectively Group), cyclophosphamide 20mg / kg positive control group (C1 and C2 group) and saline 20ml / kg negative control group (D1 and D2 group). The volume of tumor was observed. The expression of Ki67 and PCNA was detected by immunohistochemistry. Results Compared with the negative control group, the expression of Ki67 and PCNA in the Tha group and the positive control group were significantly decreased (P <0.05). In addition, the tumor volume in group A1 and the expression of Ki67 and PCNA were less than those in group A2 and B1, while those in group A2 and B1 were less than those in group B2 (P <0.05). Conclusions Tha can inhibit the growth of SMMC7721 xenografts. The anti-tumor effect of Tha is more significant than that of the control group. Thalidosis mechanism is related to the down-regulation of Ki67 and PCNA protein expression and the inhibition of tumor cell proliferation.