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目的:以休眠芽为外植体,建立党参丛生芽发生途径的实验技术体系,为党参种苗工厂化离体快繁及党参种质资源的离体保存提供理论依据和技术支持。方法:以MS为基本培养基,添加不同浓度和种类的细胞分裂素和生长素,诱导休眠芽产生丛生芽。以1/2 MS为基本培养基,添加不同浓度和种类的生长素诱导丛生芽生根。结果:MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+蔗糖30 g/L+琼脂7.5 g/L培养基是诱导休眠芽产生丛生芽及丛生芽继代培养的最佳培养基,丛生芽诱导率为50%。每个小芽丛经继代培养后可增殖12～18个新芽。最佳生根培养基是1/2 MS+IBA 2.0 mg/L+蔗糖15 g/L+琼脂7.5 g/L+活性炭1 g/L,生根率达85.7%,植株生长健壮。结论:建立了以休眠芽为外植体的党参丛生芽再生植株的离体培养体系。
OBJECTIVE: To establish dormant bud as the explant to establish the experimental technology system of Codonopsis pilosula budding pathways, providing theoretical basis and technical support for the ex vivo rapid propagation of Cotinus cogonagalli seedling factory and in vitro preservation of Cigarettes germplasm resources. Methods: With MS as the basic medium, cytokinin and auxin with different concentrations and types were added to induce dormant buds to produce cluster buds. With 1/2 MS as the basic medium, different concentration and kinds of auxin were added to induce the rooting of cluster buds. Results: MS + 6-BA 1.0 mg / L + NAA 0.5 mg / L + sucrose 30 g / L + agar 7.5 g / L medium was the best medium for inducing dormancy buds to produce subculture and clustered bud subculture. Induction rate of 50%. Each small bud after subculture can multiply 12 to 18 sprouts. The best rooting medium was 1/2 MS + IBA 2.0 mg / L + sucrose 15 g / L + agar 7.5 g / L + activated carbon 1 g / L with rooting rate 85.7%. Conclusion: The in vitro culture system of Codonopsis pilosula regeneration shoots with dormant buds as explants was established.