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目的分析研讨人血清假性胆碱酯酶连续监测法测定方法学的建立及其在肝功能评价中的应用价值。方法检测其反应初速度,选择反应体系底物用量、离子强度、最佳pH等,并评价所使用试剂稳定性和方法性能状况,进而得到结论。结果健康组和急性肝炎组对比,急性肝炎组虽有降低,但组间数据无统计学意义(P>0.05);健康组与慢性肝炎组对比,后者有明显降低(P<0.05);慢性肝炎组与肝硬变组对比,肝硬变组有明显降低(P<0.05)。结论人血清假性胆碱酯酶连续监测法操作简单,且容易配备试剂,有较好稳定性,个人建议可作为一种常规测定方式。
OBJECTIVE: To establish a methodological method for the determination of serum pseudo-cholinesterase and to evaluate its value in the evaluation of liver function. The method was used to detect the initial velocity of reaction, the amount of substrate, the ionic strength and the optimum pH of the reaction system. The stability and performance of the reagents were also evaluated. Results There was no significant difference between healthy group and acute hepatitis group (P> 0.05), but there was significant difference between healthy group and chronic hepatitis group (P <0.05). Chronic Hepatitis group and cirrhosis group, liver cirrhosis group was significantly lower (P <0.05). Conclusion Continuous monitoring of human serum pseudo-cholinesterase method is simple, and easy to use reagents, with good stability, personal recommendations can be used as a routine measurement.