根据对抗体致敏红细胞形成花环能力的不同,人类中性粒细胞可分成两种亚群。常规的中性粒细胞花环试验有两点不足之处:一是提纯中性粒细胞需较长时间;另一为提纯中性粒细胞要较多的血液。本文作者创建的一种简易、微量中性粒细胞花环试验,只需少量(<100μl)肝素抗凝全血。操作中不必提纯中性粒细胞,且整个试验过程在1 According to the ability of antibody-sensitized erythrocytes to form rosette, human neutrophils can be divided into two subgroups. Conventional neutrophil rosette test has two deficiencies: First, purification of neutrophils takes a long time; the other for the purification of neutrophils to more blood. The authors of this article created a simple, micro-neutrocytic rosette test that requires only small amounts (<100 μl) of heparin anticoagulant whole blood. Neutrophils do not need to be purified during the operation, and the entire test procedure is at 1