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目的了解浙江省部分山区蜱中感染斑点热群立克次体的状况,探讨以外膜蛋白A(rOmpA)和柠檬酸合成酶基因(gltA)为靶基因的PCR方法可行性。方法利用PCR方法,检测天台县左溪镇和临安县西天目地区共46组蜱类标本中rOmpA和gltA基因特异片段。对所检测到的阳性结果进行克隆与序列测定,并进行聚类分析。结果从46组蜱标本中检测发现2组长角血蜱中斑点热群立克次体的rOmpA和gltA基因片段均为阳性,核酸序列基本一致,但推测斑点热群立克次体种的进化位置存在差异。结论浙江省部分山区存在蜱中感染斑点热群立克次体的状况。利用rOmpA和gltA基因能从标本中检测到斑点热群立克次体,但从2个结果推测立克次体的分类位置存在差异。
Objective To investigate the status of spotted fever group Rickettsia in ticks in some mountainous areas in Zhejiang Province and to explore the feasibility of using PCR methods targeting rOmpA and gltA as target genes. Methods The specific fragments of rOmpA and gltA in 46 ticks from ZuoXi town of Tiantai county and Xitian district of Lin’an county were detected by PCR method. The detected positive results were cloned and sequenced, and cluster analysis. Results The results showed that rOmpA and gltA gene fragments of rhesus hotspot were positive in two groups of ticks, and the nucleic acid sequences were basically the same. However, the evolution of Rickettsia speciosa There are differences in location. Conclusion The status of spotted fever group Rickettsia in ticks is found in some mountainous areas of Zhejiang Province. Species of rodentia rickettsi were detected from rOmpA and gltA genes. However, the rickettsia was classified according to the two results.