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以８种方法提取烤后烟叶ＤＮＡ，测定其浓度和纯度，结合叶绿体不编码蛋白序列和３５Ｓ启动子序列的ＰＣＲ扩增结果来分析评价提取ＤＮＡ的质量，同时讨论了这些提取方法的可靠性及所提取ＤＮＡ的纯度和产量。
适于PCR分析的烤后烟叶DNA提取方法的研究
【摘 要】
：
以８种方法提取烤后烟叶ＤＮＡ，测定其浓度和纯度，结合叶绿体不编码蛋白序列和３５Ｓ启动子序列的ＰＣＲ扩增结果来分析评价提取ＤＮＡ的质量，同时讨论了这些提取方法的可靠性及所提取ＤＮＡ的纯度和产量。
【机 构】
：
黑龙江省烟草科学研究所
【出 处】
：
中国烟草科学
【发表日期】
：
1999年4期
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