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目的了解KI多瘤病毒(karolinska institutet polyomavirus,KIPyV)在我国福州儿童呼吸道感染中的检出情况及与呼吸道疾病的关系,并对毒株进行全基因组序列测定和种系分析。方法收集2007年11月~2014年3月在福建省福州市妇幼保健院因呼吸道感染住院的重症监护病房(PICU)的235例小儿鼻咽抽取物样本,通过巢式PCR扩增法用2对特异引物对KIPyV的VP1基因片段进行检测。对检测出的1株KIPyV(FZ52)用4对全基因序列引物进行扩增和拼接,获得该株病毒的全基因组序列,上传GenBank并与基因库中国外其他8株KIPyV的全基因组序列和氨基酸序列进行比对,并做种系分析。结果从中发现1例KIPyV感染阳性病例,检测阳性率为0.4%,没有检测出KIPyV与其他呼吸道病毒的混合感染,如甲、乙型流感病毒(influenza type A or B virus,Flu A or B)、呼吸道合胞病毒(respiratory syncytial virus,RSV)、人偏肺病毒(human metapnumovirus,HMPV)、人博卡病毒(human bocavirus,HBoV)、WU多瘤病毒(WU polyomavirus,WUPyV)等。与参考株斯德哥尔摩的S60相比,有8个核苷酸不同,推导出的氨基酸序列有3处不同。同时,也将该序列与国内毒株的部分核苷酸序列进行比对分析。FZ52株与斯德哥尔摩的S380株关系较近,FZ52株、S380株与聚成一簇的S350株和布里斯班的B003株关系较近。结论种系分析显示中国福建福州的FZ52株与瑞典的S380株关系最近。
OBJECTIVE To investigate the detection of KIPVV in children with respiratory infection in Fuzhou, China, and its relationship with respiratory diseases. The genome-wide sequence analysis and phylogenetic analysis of KI were performed. Methods A total of 235 pediatric nasopharyngeal aspirates collected from the intensive care unit (PICU) hospitalized for respiratory tract infection in Fuzhou MCH from November 2007 to March 2014 were collected. Two pairs of nasopharyngeal samples were collected by nested PCR. Specific primers were used to detect the VP1 gene fragment of KIPyV. One KIPyV (FZ52) detected was amplified and spliced with four pairs of whole genome primers to obtain the complete genome sequence of the strain. The full-length sequence of GenBank was cloned and linked with the genome-wide sequence of eight KIPyV genomes and amino acids Sequence alignment and phylogenetic analysis. Results One case of positive KIPyV infection was found with a positive rate of 0.4%. No mixed infection of KIPyV with other respiratory viruses such as influenza A or B virus, Respiratory syncytial virus (RSV), human metapnumovirus (HMPV), human bocavirus (HBoV), WU polyomavirus (WUPyV) and the like. Compared with S60 of reference strain Stockholm, there are 8 different nucleotides and deduced 3 different amino acid sequences. At the same time, we also compared the sequence with some nucleotide sequences of domestic strains. The strain FZ52 has a close relationship with the strain S380 in Stockholm. The strains FZ52 and S380 have a close relationship with the cluster of S350 and the strain B003 in Brisbane. Conclusion The phylogenetic analysis shows that the FZ52 strain in Fuzhou, Fujian Province of China has the closest relationship with the S380 strain in Sweden.