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目的:探讨si RNA下调AEG-1表达对乳腺癌细胞增殖和凋亡的影响。方法:用设计合成的AEG-1 siRNA转染人乳腺癌MCF-7细胞株,采用荧光定量RT-PCR技术观察AEG-1 siRNA对MCF-7细胞AEG-1 mRNA表达的影响;MTT法检测AEG-1 siRNA对MCF-7细胞增殖的抑制作用;流式细胞术检测AEG-1 siRNA对MCF-7细胞凋亡和细胞周期的影响。结果:AEG-1 siRNA转染人乳腺癌MCF-7细胞后能显著下调AEG-1基因的表达,与对照组比较差异有统计学意义(P<0.01);AEG-1基因表达的下调可以显著抑制MCF-7细胞的增殖,与对照组比较差异有统计学意义(P<0.01),促进MCF-7细胞凋亡,与对照组比较差异有统计学意义(P<0.01),并将细胞周期阻滞在G0/G1期,与对照组比较差异有统计学意义(P<0.01)。结论:AEG-1 si RNA可下调乳腺癌MCF-7细胞AEG-1基因的表达,抑制其增殖,并促进其凋亡。
Objective: To investigate the effect of si RNA on the proliferation and apoptosis of breast cancer cells induced by AEG-1. Methods: AEG-1 siRNA was transfected into human breast cancer cell line MCF-7 and the effect of AEG-1 siRNA on AEG-1 mRNA expression was detected by real-time fluorescence quantitative RT-PCR. AEG -1 siRNA on the proliferation of MCF-7 cells. The effect of AEG-1 siRNA on the apoptosis and cell cycle of MCF-7 cells was detected by flow cytometry. Results: AEG-1 siRNA could significantly down-regulate the expression of AEG-1 gene in MCF-7 cells compared with the control group (P <0.01). The AEG-1 gene expression was significantly down-regulated (P <0.01), and promoted the apoptosis of MCF-7 cells. Compared with the control group, the difference was statistically significant (P <0.01), and the cell cycle Blocking in G0 / G1 phase, compared with the control group, the difference was statistically significant (P <0.01). Conclusion: AEG-1 si RNA can down-regulate the expression of AEG-1 gene in breast cancer MCF-7 cells, inhibit its proliferation and promote its apoptosis.