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通过建立大鼠肝细胞的铜胁迫模型,旨在讨论不同剂量的铜对大鼠肝细胞活性、自由基代谢及细胞膜电位的影响。以硫酸铜为试验铜源,6个不同铜质量浓度(以Cu2+计):0,50,100,200,400,800mg/L,分别加入培养液培养BRL-3A细胞24和48h后,测定不同Cu2+质量浓度、不同处理时间后细胞自由基产生、抗氧化酶活性及细胞膜电位变化。结果显示,处理质量浓度低于400 mg/L时,细胞增殖活性差异不显著(P>0.05),处理质量浓度为400,800mg/L时,细胞活性下降(P<0.05);处理质量浓度低于400 mg/L时,Cu2+质量浓度与细胞内T-SOD、GSH-Px、CP活性呈正相关,与MDA、NO含量呈负相关,质量浓度达400mg/L及以上质量浓度时,结果相反。随着Cu2+质量浓度的增加,细胞膜电位下降(P<0.05)。以上结果表明,适量的铜含量对细胞内的抗氧化酶活性具有促进作用,但高铜可能促使细胞活性下降,自由基产生增多,细胞膜电位下降。
Through establishing a model of copper stress in rat hepatocytes, we aimed to discuss the effects of different doses of copper on the activity of hepatocytes, free radical metabolism and cell membrane potential in rats. Copper sulfate was used as the test copper source, and six different copper concentrations (Cu2 +): 0,50,100,200,400,800 mg / L were added into culture medium to culture BRL-3A cells for 24 and 48 hours respectively. The effects of different Cu2 + After free radical generation, antioxidant enzyme activity and membrane potential changes. The results showed that there was no significant difference in cell proliferation when the concentration was lower than 400 mg / L (P> 0.05), and the cell viability decreased when the concentration was 400 and 800 mg / L (P <0.05) At concentration of 400 mg / L, the concentration of Cu2 + was positively correlated with the activities of T-SOD, GSH-Px and CP in cells, but negatively correlated with the contents of MDA and NO. The results were opposite when the concentration was 400mg / L and above. With the increase of Cu2 + concentration, the cell membrane potential decreased (P <0.05). The above results show that the appropriate amount of copper can promote the activities of antioxidant enzymes in the cells, but high copper may decrease the cell viability, increase the free radicals and decrease the cell membrane potential.