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以生长在非控温控光下的４个冬性甘蓝型油菜（ＢｒａｓｓｉｃａｎａｐｕｓＬ．）品种为供体，进行游离小孢子培养。研究发现，多数品种在开花３－７天取材最为适宜。在甘蓝型油菜小孢子培养中只有单核晚期的小孢子才可能发育成胚状体，而花药培养时处于单核早期的小孢子易于发育成胚状体。在适当花期选取发育比较一致的单核晚期小孢子培养，经数小时后，部分小孢子便开始膨大，这是小孢子发育成胚的最早标志，膨大的小孢子中，有部分形成多细胞球并进一步发育成胚。用春性甘蓝型油菜为材料进行蔗糖浓度的实验结果表明：培养３天后，在１６％蔗糖培养基中存活的小孢子最多，达１６．１３％；培养３０天后，胚状体诱导频率则以１３％蔗糖浓度为最高，每花蕾可达１４４个胚状体。如果在１６％蔗糖培养基中培养３天后，添加等体积的１３％蔗糖培养基，能够大大提高胚状体的诱导频率，为仅用１３％蔗糖培养基培养的３．７倍。这一实验体系正在用于抗菌核病的诱变与筛选，并作为外源基因导入的实验体系。
Four winterbred Brassica napus L. varieties grown under non-temperature-controlled light conditions were used as donors for free microspore culture. The study found that most varieties in the flowering 3-7 days most suitable material. In the microspore culture of Brassica napus, only the microspore of the late stage of mononuclear could develop into the embryoid body. However, the microspores in early mononuclear stage of anther culture were easy to develop embryoid body. At the appropriate flowering stage, the microspore culture in late stage of mononuclear stage with the same development was chosen. After a few hours, some microspores began to expand, which was the earliest sign of microspore development into embryos. Some of the expanded microspores formed multicellular spheres And further develop into embryos. The experimental results of sucrose concentration in spring rapeseed showed that the number of microspores survived in 16% sucrose medium reached 16.13% after 3 days of culture, and the induction frequency of embryoid body after 30 days The 13% sucrose concentration was the highest, reaching 144 embryoids per bud. If an equal volume of 13% sucrose medium is added after 3 days of culture in 16% sucrose medium, the frequency of embryoid induction can be greatly increased, 3.7 times more than that cultured with 13% sucrose alone. This experimental system is being used for mutagenesis and screening of antibacterial sclerosis and as an experimental system for the introduction of foreign genes.