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目的:观察电刺激大鼠黑质网状部(substantia nigra pars reticulata,SNr)对脚桥核(pedunculopontine nu-cleus,PPN)神经元自发放电活动的影响,进一步探讨脑内电刺激治疗帕金森病(Parkinson’s disease,PD)的机制。方法:应用细胞外记录方法观察不同频率电刺激大鼠SNr对PPN神经元自发放电的影响。结果:实验记录了大鼠46个PPN神经元的自发放电,其放电频率在2.4~55.4 Hz之间,平均为(21.3±13.8)Hz;低频电刺激(5~10Hz)大鼠SNr时,PPN神经元的自发放电无明显变化(P>0.05),随着刺激频率(20~200 Hz)的增加,大多数PPN神经元的自发放电活动受抑制,神经元放电频率较刺激前明显降低,且在100 Hz时的抑制作用最强(P<0.05)。结论:高频电刺激大鼠SNr对PPN神经元自发放电的影响主要为抑制,提示高频电刺激SNr可通过抑制PPN神经元活动参与PD的治疗。
Objective: To investigate the effect of electrical stimulation of substantia nigra pars reticulata (SNr) on spontaneous discharges of pedunculopontine nu-cleus (PPN) neurons in rats and further explore the effects of electrical stimulation of brain electrical stimulation on Parkinson’s disease (Parkinson’s disease, PD) mechanism. Methods: The effects of SNr stimulation at different frequencies on spontaneous firing of PPN neurons were observed by extracellular recording method. RESULTS: The spontaneous discharges of 46 PPN neurons in rats were recorded. The firing rate was between 2.4 and 55.4 Hz with an average of (21.3 ± 13.8) Hz. In the SNr of low frequency electrical stimulation rats (5 to 10 Hz), PPN The spontaneous discharge of neurons showed no significant change (P> 0.05). With the increase of stimulation frequency (20 ~ 200 Hz), the spontaneous discharge activities of most PPN neurons were inhibited, and the discharge frequency of neurons was significantly lower than that before stimulation The inhibition was the strongest at 100 Hz (P <0.05). Conclusion: The effect of SNr on spontaneous discharge of PPN neurons is mainly inhibited by high frequency electrical stimulation of SNr, suggesting that high frequency electrical stimulation of SNr can participate in the treatment of PD by inhibiting the activity of PPN neurons.