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目的:检测CD4+CD25-Foxp3+T细胞在系统性红斑狼疮(SLE)患者外周血中的表达,探讨该细胞亚群在SLE发病机制中的意义。方法:采用流式细胞术检测25例SLE患者和15例健康对照的外周血CD4+CD25-Foxp3+T细胞比例、分析其表型;利用免疫磁珠细胞分选法(MACS)分选出CD4+CD25-T细胞,通过实时定量聚合酶链反应(real-timePCR)检测FOXP3基因表达。结果:活动期和稳定期SLE患者外周血CD4+CD25-Foxp3+T细胞比例分别是:(9.89±0.85)%和(7.11±0.86)%,与健康对照组(3.35±0.31)%比较差异有统计学意义(P<0.01)。SLE患者CD4+CD25-Foxp3+T细胞表达频率与SLEDAI评分呈明显正相关(P=0.0008)。活动期SLE患者CD4+CD25-T细胞中Foxp3在蛋白和基因转录水平上的表达均高于健康对照组(P<0.01)。SLE患者CD4+CD25-Foxp3+T细胞表面低表达CD127。结论:SLE患者外周血中CD4+CD25-Foxp3+T细胞比例变化可能反应了SLE发病中T细胞外周免疫失耐受机制“调节性T细胞的抑制效应被抵抗”。
Objective: To detect the expression of CD4 + CD25-Foxp3 + T cells in the peripheral blood of patients with systemic lupus erythematosus (SLE) and to explore the significance of this cell subpopulation in the pathogenesis of SLE. Methods: The proportion of CD4 + CD25-Foxp3 + T cells in peripheral blood of 25 patients with SLE and 15 healthy controls was detected by flow cytometry. The phenotype of CD4 + CD25-Foxp3 + T cells was analyzed by using flow cytometry. CD4 + CD25-T cells, FOXP3 gene expression was detected by real-time PCR. Results: The percentage of CD4 + CD25-Foxp3 + T cells in active and stable SLE patients was (9.89 ± 0.85)% and (7.11 ± 0.86)%, respectively, compared with 3.35 ± 0.31% in healthy controls Statistical significance (P <0.01). The frequency of CD4 + CD25-Foxp3 + T cells expression in SLE patients was positively correlated with SLEDAI score (P = 0.0008). The expression of Foxp3 at the transcriptional level of protein and gene in CD4 + CD25-T cells of active SLE patients was higher than that of healthy controls (P <0.01). SLE patients with low expression of CD127 on the surface of CD4 + CD25-Foxp3 + T cells. Conclusion: The changes of the proportion of CD4 + CD25-Foxp3 + T cells in peripheral blood of patients with SLE may reflect the mechanism of immune tolerance of peripheral T cells in peripheral blood of SLE patients and that “the inhibitory effect of regulatory T cells is resisted.”