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目的采用动物肿瘤模型探讨2硝基咪唑卟啉二乙撑三氨四乙酸(MISO卟啉DTTA)的放射增敏效应。方法将移植有Lewis肺癌的C57BL/6小鼠随机分为7个组:空白对照组、乏氧对照组(阻断小鼠荷瘤肢体血流10min)、单纯用药组(分为8.58、17.15mg/kg体重2个亚组腹腔内单次注射给药)、单纯放射组(分为7.5、15.0、25.0Gy3个亚组单次照射)、乏氧放射组(乏氧条件下分为7.5、15.0、25.0Gy3个亚组单次照射)、放射+药物组(分6个亚组)、乏氧放射+药物组(分6个亚组)。每组8只小鼠。采用肿瘤生长延缓方法进行放射增敏实验。结果当肿瘤体积达到实验开始时体积4倍时,分别测得8.58、17.15mg/kg体重的MISO卟啉DTTA在有氧状态下的放射增敏比平均为1.62和1.89,在乏氧状态下的平均为1.57和1.77。空白对照组、乏氧对照组、单纯用药1组和单纯用药2组的平均存活时间分别为(31.8+4.6)、(29.1+4.3)、(29.5+3.8)、(33.4+5.1)d(F=1.63,P=0.205)。结论8.58、17.15mg/kg体重的MISO卟啉DTTA对Lewis肺癌具有放射增敏效应,且对小鼠无毒性。
Objective To investigate the radiosensitizing effect of 2-nitroimidazoline porphyrin diethylenetriamine tetraacetic acid (MISO porphyrin DTTA) using an animal tumor model. Methods C57BL/6 mice transplanted with Lewis lung cancer were randomly divided into 7 groups: blank control group, hypoxic control group (blocked blood flow of mice bearing tumor limbs for 10 minutes), and simple drug group (divided into 8.58, 17.15 mg). /kg body weight in two subgroups (administered intraperitoneally by single injection), radiotherapy alone (divided into 7.5, 15.0, 25.0 Gy, 3 subgroups of single irradiation), hypoxic radiation group (differentiated under hypoxic conditions 7.5, 15.0) (25.0 Gy3 subgroup single exposure), radiation + drug group (6 subgroups), hypoxic radiation + drug group (6 subgroups). 8 mice per group. Radiosensitization experiments were performed using tumor growth delay methods. Results When the tumor volume reached 4 times the initial volume of the experiment, the radiosensitization ratios of 8.58 and 17.15 mg/kg body weight of MISO porphyrin DTTA under aerobic conditions were 1.62 and 1.89, respectively, under hypoxic conditions. The average is 1.57 and 1.77. The average survival time of blank control group, hypoxic control group, simple drug 1 group and simple drug 2 group were (31.8+4.6), (29.1+4.3), (29.5+3.8), (33.4+5.1)d (F, respectively). =1.63, P=0.205). Conclusions 8.58 and 17.15 mg/kg body weight of MISO porphyrin DTTA have radiosensitizing effect on Lewis lung cancer and have no toxicity to mice.