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以热研Ⅰ号为广亲和基因(S_5~n)的供体亲本,粳型雄性不育保持系76—27B作为轮回亲本,用回交和测交鉴定同步进行的方式,将S_5~n基因逐步导人到76—27B中。在BC_3F_3后代中选得8份材料,其中5份材料73-2-1、73-2-4、73-2-6、73-2-7和73-2-8带有S_5~n基因,以下简称B(S_5~n S_5~n);另3份材料73-2-2、73-2-3和73-2-5带有S_5~J基因,简称B(S_5~J S_5~J)。亲和性比较分析表明:B(S_5~n S_5~n)的亲和性极显著地高于76-27B及B(S_5~J S_5~J),但同时又低于供体亲本热研Ⅰ。回交转育成的B(S_5~n S_5~n)的亲和谱小于热研Ⅰ,前者与籼稻品种南京11、华联8号、珍汕97 B和浙农8010的杂种平均结实率分别为69.78％(正常)、76.62％(正常)、44.42％(半不育)和44.36％(半不育);而后者与上述4个品种的杂种结实率均表现正常,分别为82.1％、67.2％、79.1％和73.8％。B(S_5~J S_5~J)、76-27 B与上述4个品种杂交,杂种均表现为半不育或高不育,平均育性范围为20.03％～56.70％,广亲和基因S_5~n的导入,可使籼粳杂种的结实率在原有基础上提高26.63％左右。
The donor progenies with a broad compatibility gene (S_5 ~ n) and the japonica CMS line 76-27B were selected as the recurrent parents by backcrossing and crossbreeding identification. The S_5 ~ n Gene gradually guided to 76-27B. Among the BC_3F_3 progeny, 8 were selected, of which 5 were 73-2-1, 73-2-4, 73-2-6, 73-2-7 and 73-2-8 with S_5 ~ n genes. Referred to B (S_5 ~ n S_5 ~ n); the other three materials 73-2-2, 73-2-3 and 73-2-5 with S_5 ~ J gene, referred to as B (S_5 ~ J S_5 ~ J). Affinity analysis showed that the affinity of B (S_5 ~ n S_5 ~ n) was significantly higher than that of 76-27B and B (S_5 ~ J S_5 ~ J), but lower than that of donor parent . The affinity spectrum of B (S_5 ~ n S_5 ~ n) which was backcrossed and regenerated was smaller than that of ThermoF.Ⅰ, and the average seed set rate of hybrids of former and indica rice varieties Nanjing 11, Hualian 8, Zhenshan 97 B and Zheonong 8010 was 69.78% (normal), 76.62% (normal), 44.42% (semi-sterile) and 44.36% (semi-sterile) respectively. The seed set rate of the latter and the above four varieties all showed normal, , 79.1% and 73.8% respectively. B (S_5 ~ J S_5 ~ J), 76-27 B crossed with the above four breeds. The hybrids showed semi-sterility or high sterility. The average fertility ranged from 20.03% to 56.70% The introduction of n could make the seed setting rate of indica-japonica hybrids increase by 26.63% on the original basis.