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目的探讨P53,CyclinD1和Ki67蛋白在外阴HPV相关疾病尖锐湿疣、外阴上皮内瘤变、外阴鳞状细胞癌中的表达及意义。方法采用免疫组化SP法检测30例尖锐湿疣;60例外阴上皮内瘤变;20例外阴鳞状细胞癌标本中P53,CyclinD1,Ki67的表达情况,并与10例正常组织进行对照。结果 P53在尖锐湿疣组,外阴上皮内瘤变(VINⅠ级、Ⅱ级、Ⅲ级)组,外阴鳞状细胞癌组,正常组的阳性表达率分别为63.66%,43.48%,52.63%,61.11%,70.00%和0。CyclinD1的阳性表达率分别为43.33%,34.78%,47.37%,55.56%,60.00%和0。Ki67的阳性表达率分别为46.67%,47.83%,57.89%,72.22%,85.00%和0。P53与Ki67蛋白阳性表达呈正相关(P<0.05),Ki67与CyclinD1蛋白阳性表达呈正相关(P<0.05),P53与CyclinD1蛋白阳性表达无相关性(P>0.05)。三者均表达随外阴上皮恶变的进展而增加。结论 P53,CyclinD1和Ki67蛋白在外阴HPV相关疾病的发生,发展中起一定作用。
Objective To investigate the expression and significance of P53, CyclinD1 and Ki67 in genital HPV-related condylomata acuminata, vulvar intraepithelial neoplasia and vulvar squamous cell carcinoma. Methods Immunohistochemical SP method was used to detect the expression of P53, CyclinD1, Ki67 in 30 cases of genital warts, 60 cases of vulvar intraepithelial neoplasia and 20 cases of vulvar squamous cell carcinoma specimens, and compared with 10 cases of normal tissues. Results The positive expression rate of P53 in condyloma acuminata group, vulvar intraepithelial neoplasia (VINⅠ grade, Ⅱ grade, Ⅲ grade) group, vulvar squamous cell carcinoma group and normal group were 63.66%, 43.48%, 52.63%, 61.11% , 70.00% and 0. The positive rates of CyclinD1 were 43.33%, 34.78%, 47.37%, 55.56%, 60.00% and 0 respectively. The positive rates of Ki67 were 46.67%, 47.83%, 57.89%, 72.22%, 85.00% and 0 respectively. There was a positive correlation between the expression of P53 and Ki67 protein (P <0.05), the positive expression of Ki67 and CyclinD1 protein (P <0.05), but there was no correlation between the expression of P53 and CyclinD1 protein (P> 0.05). All three expressed with the progress of vulvar epithelial malignancies increased. Conclusion P53, CyclinD1 and Ki67 proteins play a role in the occurrence and development of vulvar HPV-related diseases.