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目的建立一种人神经生长因子(NGF)mRNA荧光定量聚合酶链反应(PCR)检测方法,探讨接触时间对正己烷接触人群NGF mRNA表达水平的影响。方法以接触正己烷工龄分组。设计引物、探针,检测对照组和接触正己烷各组之间的NGF mRNA水平。结果利用该荧光定量PCR检测结果显示工龄<2年组、2～6年组、>6年组以及对照组的NGF mRNA以10为底的对数分别为(8.88±0.08)、(8.68±0.08)、(8.53±0.11)和(8.90±0.07)copies/L。2～6年组和>6年组的NGF mRNA表达水平均低于对照组。各组内男女之间NGF mRNA表达水平比较,差异无统计学意义(P>0.05)。结论正己烷的毒性对于NGF mRNA的表达有明显的时间依赖关系,随着接触时间加长,NGF的表达逐渐减弱;所建立的检测方法特异性强,完全符合检测要求。
Objective To establish a real-time fluorescent quantitative polymerase chain reaction (PCR) assay to detect the expression of NGF mRNA in human exposure to n-hexane. Method to contact n-hexane length of service grouping. Primers, probes, NGF mRNA levels between control and n-hexane exposure groups were designed. Results The results of real-time PCR showed that the base 10 logarithm of NGF mRNA was (8.88 ± 0.08), (8.68 ± 0.08) in the group of <2 years, 2 to 6 years,> 6 years and control group ), (8.53 ± 0.11) and (8.90 ± 0.07) copies / L, respectively. The levels of NGF mRNA in 2-6-year and> 6-year groups were lower than those in control group. There was no significant difference of NGF mRNA expression between men and women in each group (P> 0.05). Conclusion The n-hexane toxicity has a significant time-dependent relationship with the expression of NGF mRNA. With the prolongation of the contact time, the expression of NGF gradually decreases. The established detection method is specific and fully meets the detection requirements.