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[目的]建立一套完善,可靠,适合深圳人群的HIV-1耐药性基因型检测方法,调查分析深圳地区未接受抗病毒治疗的HIV感染者/AIDS患者的病毒株耐药突变及其本底流行情况。[方法]从未接受抗病毒治疗的HIV-1感染者血浆中提取病毒RNA,采用RT-PCR扩增目的基因片断,并对扩增片断进行序列测定和分析。[结果]通过HIV-1耐药性基因型检测法能够获得1300bp长度的目的基因片断。经序列分析所得的21份RT基因结果中有8份对NRTI产生耐药突变(38.5%),5份对NNRTI产生耐药突变(23.81%);另外分析所得的12份PR基因结果中1份产生对蛋白酶抑制剂主要耐药突变(8.33%),5份产生对蛋白酶抑制剂次要耐药突变(41.67%)。[结论]HIV-1耐药性基因型检测法能有效地监测HIV-1感染者血浆中的耐药性病毒株的存在,并且检测结果表明,深圳地区未接受抗病毒治疗的HIV-1感染者中已经存在耐药突变株。
[Objective] To establish a complete, reliable and suitable HIV-1 resistant genotype detection method for Shenzhen population, and to investigate the drug-resistant mutations in HIV-1 infected patients / AIDS patients who did not receive antiviral therapy in Shenzhen Bottom prevalence. [Method] The viral RNA was extracted from the plasma of HIV-1-infected patients who had never received antiviral therapy. The target gene fragments were amplified by RT-PCR and the amplified fragments were sequenced and analyzed. [Results] The target gene fragment with length of 1300bp could be obtained by HIV-1 resistance genotyping assay. Eight of the 21 RT gene results obtained by sequence analysis showed resistance mutation to NRTI (38.5%) and five mutations to NNRTI (23.81%). In addition, 1 out of 12 PR gene analysis results There were major resistance mutations (8.33%) to protease inhibitors and 5 to minor resistance mutations (41.67%) to protease inhibitors. [Conclusion] The HIV-1 resistant genotype detection method can effectively monitor the presence of drug-resistant virus strains in the plasma of HIV-1 infected patients and the results show that HIV-1 infection in Shenzhen without antiviral therapy There have been drug-resistant mutant strains.