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灰飞虱的群体带毒率及体内条纹病毒(rice stripe virus,RSV)的准确检测是预测水稻条纹叶枯病在CO2浓度升高背景下能否大发生的重要参考依据之一。本研究应用quali-fiedRT-PCR(qRT-PCR)测定不同CO2浓度条件下灰飞虱体内的RSV含量。结果表明:在试验的3个CO2浓度梯度(370、470和570μl.L-1)下,470μl.L-1的CO2浓度最适合RSV在灰飞虱体内的增殖,此方法与现已普遍应用的酶联免疫检测法(enzyme-linkedi mmu-nosorbnent assay,ELISA)和斑点免疫结合检测法(dotimmunobinding assay,DIBA)相比,具有快速、灵敏和特异性强的优点,且不需制备抗血清,适于普及应用。
The accurate detection of SBPH and RSV in Laodelphax striatellus is one of the important references for predicting the occurrence of rice stripe virus (SSRI) under the background of increasing CO2 concentration. In this study, the RSV content in the SBPH was determined by using quali-fied RT-PCR (qRT-PCR). The results showed that under the three CO2 concentration gradients (370, 470 and 570μl.L-1), 470μl.L-1 CO2 was the most suitable for the RSV multiplication in the planthopper, and this method has been widely used Compared with the enzyme-linked immunosorbnent assay (ELISA) and dotimmunobinding assay (DIBA), it has the advantages of fast, sensitive and specific, without the need of antiserum, Suitable for universal application.