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目的 研究端粒酶活性检测在大肠癌临床诊断和确定手术切除范围中的意义。方法 应用已建立的银染TRAP方法 ,检测 2 9例大肠癌组织及癌旁 (近端即食管端距癌组织 2cm、4cm、6cm、10cm ,远端即肛门端距癌组织 2cm、4cm、6cm )黏膜组织 ,共 8个标本的端粒酶活性。结果 大肠癌组织的端粒酶活性阳性率为 89.7% ( 2 6/2 9) ,其中近端 2cm癌旁黏膜组织的阳性率为 13.8% ( 4/2 9) ,远端 2cm癌旁黏膜组织的阳性率为 3.4% ( 1/2 9)。而近端 4cm、6cm、10cm ,远端 4cm、6cm癌旁黏膜组织端粒酶活性检测阳性率均为 0 ( 0 /2 9)。端粒酶活性与大肠癌的分化程度及肠系膜淋巴结转移无相关性 (P >0 .0 5 )。结论 端粒酶活性既有可能成为大肠癌诊断的标志物 ,又可望为临床上确定手术切除范围提供一定的依据
Objective To study the significance of detecting telomerase activity in the clinical diagnosis and surgical resection of colorectal cancer. Methods The established silver staining TRAP method was used to detect the expression of MMP-9 in 29 cases of colorectal carcinoma and its adjacent tissues (2 cm, 4 cm, 6 cm and 10 cm proximal to the esophageal cancer tissue, 2 cm, 4 cm and 6 cm ) Mucosal tissue, a total of eight specimens of telomerase activity. Results The positive rate of telomerase activity in colorectal cancer tissues was 89.7% (2 6/2 9). The positive rate of 2cm proximal colorectal mucosa was 13.8% (4/2 9), and the distal 2cm colorectal mucosa The positive rate was 3.4% (1/2 9). The positive rates of telomerase activity in the adjacent mucosa of 4cm, 6cm, 10cm, 4cm, 6cm were 0 (0/29). There was no correlation between telomerase activity and the differentiation of colorectal carcinoma and mesenteric lymph node metastasis (P> 0.05). Conclusion The telomerase activity may not only be a marker for the diagnosis of colorectal cancer, but also provide a basis for the clinical determination of the scope of resection