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目的研究戊四氮点燃大鼠脑内 P-糖蛋白(P-gp)和缺氧诱导因子1α(Hif-1α)的表达变化和表达部位,探讨二者之间的关系。方法反复腹腔注射戊四氮(40 mg/kg)制备大鼠点燃模型或注射 PBS 作对照。点燃后不同时间点处死动物。用免疫组织化学染色方法检测 P-gp 和 Hif-1α的表达变化,用双标免疫酶组织化学染色法确定 P-gp 和 Hif-1α的表达部位。结果对照组的 P-gp 和Hif-1α零星分布在毛细血管内皮细胞,点燃组 P-gp 和 Hif-1α分布在海马和皮质神经元的细胞膜,均以点燃后4 h 的表达最高(P-gp:1.09±0.20;Hif-1α:1.12±0.19,F=2.83,P<0.05),且分布方式相似。结论癫癎反复发作可以诱导脑内神经元表达 P-gp 和 Hif-1α,二者可能具有相关性。
Objective To investigate the expression and location of P-glycoprotein (P-gp) and hypoxia-inducible factor-1α (Hif-1α) in brain of pentylenetetrazole-lighted rats and to explore the relationship between the two. Methods Rats were injected intraperitoneally with pentylenetetrazole (40 mg / kg) to establish lit model or PBS as control. Animals were sacrificed at different time after lighting. The expressions of P-gp and Hif-1α were detected by immunohistochemical staining. The expression sites of P-gp and Hif-1α were determined by double-stained immunohistochemical staining. Results P-gp and Hif-1α were sporadically distributed in the capillary endothelial cells in the control group, and P-gp and Hif-1α in the ignited group were distributed in the cell membrane of hippocampus and cortical neurons. The expression of P-gp and Hif- gp: 1.09 ± 0.20; Hif-1α: 1.12 ± 0.19, F = 2.83, P <0.05). Conclusion Recurrent epilepticus can induce the expression of P-gp and Hif-1α in the neurons of the brain, which may be related to each other.