临床上,拉米夫定在获得显著的抑制HBV复制的同时,亦可诱发高比例,多位点的耐药突变。目前常规的检测方法,如PCR、单链多态性等检测技术,均不能一次性检出多位点的基因突变,DNA测序能有效检出多位点突变,但过程复杂,不易用于临床检测。基因芯片技术具有操作简单化和多位点的平行检测特点,能较好地解决临床应用的问题。为此,我们针 Clinically, lamivudine can significantly inhibit HBV replication at the same time, can also induce high-rate, multi-site resistance mutations. Currently, conventional detection methods, such as PCR and single-stranded polymorphism, can not detect multi-locus mutations at one time. DNA sequencing can effectively detect multi-locus mutations, but the process is complex and not easy to be used in clinical practice Detection. Gene chip technology with simple operation and multi-site parallel detection characteristics, can better solve the clinical application of the problem. To this end, we needle