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在致突变试验代谢活性研究中,制备肝S 9必须用苯巴比妥钠等物质处理动物,以诱导单加氧酶系统。Brown等报道,乙醚是一种肝细胞色素P 450包括单加氧酶系统的诱导剂和体外微粒体药物代谢的抑制剂。作者采用小鼠乙醚吸入浅麻醉同时注射苯巴比妥钠连续三或四天,研究麻醉次数对肝S 9的氨基比林和对硝基茴香醚脱甲基酶、脂质过氧化作用和酶稳定性的影响。并研究其有关遗传作用。实验小鼠先吸入乙醚直至熟睡(约1分钟),然后以苯巴比妥钠(100 mg/kg,仅第二
In the mutagenicity test metabolic activity studies, the preparation of liver S9 must be treated with phenobarbital sodium and other substances to induce the monooxygenase system. Brown et al. Reported that diethyl ether is an inducer of hepatocyte cytochrome P450 including the monooxygenase system and an inhibitor of in vitro microsomal drug metabolism. The author used mouse ether inhalation light anesthesia while injecting phenobarbital sodium for three or four consecutive days to study the number of anesthesia on hepatic S 9 aminopyrine and p-nitroanisole demethylase, lipid peroxidation and enzyme The impact of stability. And study its genetic role. The mice were aspirated with diethyl ether until asleep (about 1 minute) and then treated with phenobarbital (100 mg / kg, second only