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目的：为了探讨中华眼镜蛇毒（ＮＮＡＶ）对荷人鼻咽癌（ＮＰＣ）裸鼠血浆丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）活性的影响。方法：采用比色法检测经腹腔注射低、中或高浓度ＮＮＡＶ（１μｇ／ｍｌ、５μｇ／ｍｌ、１０μｇ／ｍｌ）的荷人ＮＰＣ裸鼠血浆ＭＤＡ和ＳＯＤ活性。结果：荷瘤鼠血浆ＭＤＡ（８４２ｎｍｏｌ／ｍｌ）比正常裸鼠（３９２ｎｍｏｌ／ｍｌ）明显升高，而荷瘤鼠血浆总ＳＯＤ（Ｔ－ＳＯＤ）、锰ＳＯＤ（Ｍｎ－ＳＯＤ）和铜锌ＳＯＤ（ＣｕＺｎ－ＳＯＤ）活性（５２１９Ｎｕ／ｍｌ，２０３１Ｎｕ／ｍｌ，３１８８Ｎｕ／ｍｌ）比正常裸鼠（１２５６４Ｎｕ／ｍｌ，７５．１１Ｎｕ／ｍｌ，５０．５３Ｎｕ／ｍｌ）低；低、中和高浓度ＮＮＡＶ均可使荷瘤鼠血浆ＭＤＡ降低或至正常范围（５６２ｎｍｏｌ／ｍｌ，５．１７ｎｍｏｌ／ｍｌ，４．５８ｎｍｏｌ／ｍｌ），并可使荷瘤鼠Ｔ－ＳＯＤ、Ｍｎ－ＳＯＤ和ＣｕＺｎ－ＳＯＤ有不同程度的升高，其中以高浓度组为佳（１０８８８Ｎｕ／ｍｌ，７２２７Ｎｕ／ｍｌ，３６６１Ｎｕ／ｍｌ，）。结论：结果提示ＮＮＡＶ的抑瘤作用可能与其降低荷瘤鼠自由基水平和提高ＳＯＤ活性有关。
Objective: To investigate the effect of Naja naja atra Venom (NNAV) on malondialdehyde (MDA) and superoxide dismutase (SOD) activity in nude mice bearing human nasopharyngeal carcinoma (NPC). Methods: Colorimetric assay was used to detect the plasma MDA and SOD activity in nude mice bearing human NPAV (1μg/ml, 5μg/ml, 10μg/ml) injected intraperitoneally at low, medium or high concentrations. Results: The plasma MDA (842nmol/ml) in tumor-bearing mice was significantly higher than that in normal nude mice (392nmol/ml), while the total SOD (T-SOD) and manganese-SOD (Mn-SOD) in plasma of tumor-bearing mice. Copper-zinc SOD (CuZn-SOD) activity (5219 Nu/ml, 2031 Nu/ml, 3188 Nu/ml) was higher than normal nude mice (12564 Nu/ml, 75.11 Nu/ml, 50.53 Nu/ml )Low; low, medium and high concentrations of NNAV can reduce the plasma MDA in tumor-bearing mice to normal range (562 nmol/ml, 5.17 nmol/ml, 4.58 nmol/ml), and can make tumor mice T - SOD, Mn-SOD, and CuZn-SOD increased in varying degrees, with the high concentration group being preferred (108 88 Nu/ml, 72 27 Nu/ml, 36 61 Nu/ml,). Conclusion: The results suggest that the anti-tumor effect of NNAV may be related to the decrease of free radical levels and increase of SOD activity in tumor-bearing mice.