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目前广泛用来诊断心肌梗塞和估计梗塞大小的非创伤性方法是:酶学测定(如测定CPK,GOT,和LDH)或肌球蛋白放射免疫学测定。但这些方法大多数的敏感性和特异性不高,仅以肌酸磷酸激酶(CPK)为例,近来有人报告,梗塞面积越大,从梗塞中心流出的CPK就越少,用常规的分析方法就会低估了大面积的心肌梗塞。作者报告了一种心肌肌凝蛋白的小分子亚单位-LCⅡ(轻链Ⅱ的敏感的放射免疫测定法,即在梗塞后测定血清中LCⅡ水平,用来诊断心肌梗塞并估计梗塞的大小。先将狗或人的左室心肌肌凝蛋白提取并纯化,
Current non-invasive methods widely used to diagnose myocardial infarction and to estimate infarct size are: enzymatic assays (eg, determination of CPK, GOT, and LDH) or myosin radioimmunoassay. However, these methods most of the sensitivity and specificity is not high, only to creatine phosphokinase (CPK), for example, has recently been reported, the greater the infarct size, outflow from the infarction center CPK less, using conventional analytical methods Will underestimate the large area of myocardial infarction. The authors report a sensitive small-molecule subunit of cardiac myosin-LCII (sensitive radioimmunoassay for light chain II, which measures serum LCII levels after infarction and is used to diagnose myocardial infarction and assess infarct size. The dog or human left ventricular myocardium myosin was extracted and purified,