论文部分内容阅读
本文通过测定P388D_1细胞的吞噬作用和Fc受体,观察其免疫功能.它具有巨噬细胞相似的吞噬功能.可吞噬经兔抗鸡红细胞(CRBC)抗体调理的CRBC,吞噬率和吞噬指数受到抗体稀释度的影响.在稀释度1∶1500时,吞噬率和吞噬指数分别为90%、4.3.经EA玫瑰花环试验证明P388D_1细胞膜表面存有Fc受体,它的花环形成率为90%.这说明它是一个较均一的细胞群.并可用于巨噬细胞在体外免疫功能研究中作为替代物.
In this paper, the phagocytosis and Fc receptor of P388D_1 cells were measured to observe their immune function.It has similar phagocytic function of macrophages.The phagocytosis of CRBC by rabbit anti-chicken erythrocyte (CRBC) antibody phagocytosis rate and phagocytic index by antibody Dilution of the phagocytosis rate and phagocytic index were 90%, respectively, at a dilution of 1: 1500, 4.3. The rosettes of the P388D_1 cell membrane on the surface of the P388D_1 cell proved to have an Fc receptor whose rosette formation rate was 90% by EA rosette assay. Indicating that it is a more homogeneous cell population and can be used as a surrogate for macrophages in in vitro immune function studies.