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目的:探索用TNF-α基因修饰联合冷冻法制备更高效能的抗肝癌疫苗的可能性。方法:分别用冷冻和60Co照射处理或者单独用60co照射处理TNF-α基因修饰的H22细胞,分别制备疫苗。比较这2种疫苗的抗小鼠接种性肝癌的作用。结果:用冷冻和60Co照射处理TNF-α基因修饰的H22细胞制成的疫苗,其提高小鼠的抑瘤率、脾淋巴细胞IL-2和TNF诱生水平及脾脏NK细胞杀伤活性的作用,均明显优于单独用60Co照射处理TNF-α基因修饰的H22细胞制成的疫苗。结论:冷冻处理TNF-α基因修饰的H22细胞,可提高该细胞的免疫原性,从而可提高疫苗的抗小鼠接种性肝癌的效能。
Objective: To explore the possibility of using TNF-α gene modification combined with freezing method to prepare more efficient anti-HCC vaccine. Methods: The TNF-α gene-modified H22 cells were treated with 60Co irradiation or 60Co irradiation alone, respectively, to prepare vaccines. Compare the effect of these two vaccines against mouse-inoculated HCC. Results: The vaccine prepared by treatment of TNF-α gene modified H22 cells with frozen and 60Co irradiation enhanced the inhibitory rate of tumor, the level of IL-2 and TNF induced by splenic lymphocytes and the activity of spleen NK cells. Were significantly better than the 60Co irradiation alone TNF-α gene modified H22 cells made vaccine. Conclusion: Cryoprecipitation of TNF-α gene-modified H22 cells can enhance the immunogenicity of the cells and thus enhance the efficacy of the vaccine against mouse-inoculated HCC.