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目的 :观察低氧预处理对大鼠海马神经元缺氧耐受性和白细胞介素 1β(IL 1β)表达的影响。 方法 :取培养 12d的两组 (对照组和低氧预处理组 )培养神经元 ,同时置于缺氧环境 (0 .9L/LN2 ,0 .1L/LCO2 )中培养 2、4、8和 12h。分别观察它们的形态变化和神经元存活数 ,并用抗rhIL 1β单克隆抗体进行免疫组织化学染色 ,观察缺氧对大鼠海马培养神经元IL 1β表达的影响。 结果 :经低氧预处理的海马神经元缺氧后IL 1β表达较对照组减弱 ,神经元损伤程度减轻 ,神经元存活数明显高于对照组。结论 :低氧预处理可使海马培养神经元对缺氧产生耐受 ,其中IL 1β表达降低可能是海马神经元对缺氧的一种适应性变化
AIM: To observe the effects of hypoxic preconditioning on hypoxic tolerance and interleukin 1β (IL 1β) expression in hippocampal neurons of rats. Methods: Neurons were cultured in both groups (control group and hypoxic preconditioning group) for 12 days and cultured for 2, 4, 8, and 12 h in hypoxia environment (0.9L / LN2, 0.1L / LCO2) . Their morphological changes and neuron survival numbers were observed respectively. Immunohistochemical staining was performed with anti-rhIL 1β monoclonal antibody to observe the effect of hypoxia on the expression of IL-1β in cultured hippocampal neurons of rats. Results: After hypoxia, the expression of IL-1β in hippocampal neurons preconditioned with hypoxia was weaker than that in the control group, the degree of neuronal damage was reduced and the number of neurons survived was significantly higher than that of the control group. CONCLUSION: Hypoxic preconditioning can make hippocampal neurons tolerant to hypoxia, and the decrease of IL 1β expression may be an adaptive change of hippocampal neurons to hypoxia