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目的:探讨大黄总蒽醌含药血清对体外培养LoVo细胞的水通道蛋白基因(AQP4)表达的调节效应。方法:16只SD大鼠随机分为正常组,大黄总蒽醌低、中、高剂量组(1.4,2.5,4.5 g.kg-1.d-1,灌胃),7 d后麻醉处死取血制备含药血清。体外培养LoVo细胞并给予不同剂量大黄总蒽醌含药血清培养液24 h,采用W estern b lot及半定量RT-PCR检测AQP4蛋白及mRNA的表达。结果:结果显示,4.5 g.kg-1.d-1大黄总蒽醌含药血清培养液可抑制LoVo细胞的AQP4蛋白及mRNA的表达(P<0.01)。结论:大黄总蒽醌含药血清可抑制LoVo细胞AQP4基因转录与翻译,提示大黄的泻下作用可能与调节AQP4表达有关。
OBJECTIVE: To investigate the regulation of aquaporin gene (AQP4) expression in cultured LoVo cells cultured in vitro by rhizome-containing serum of Rhubarb. METHODS: Sixteen SD rats were randomly divided into normal group, low-, medium-, and high-dose group of rhubarb (1.4, 2.5, 4.5 g.kg-1.d-1, gavage). After 7 days, the rats were killed. Blood preparation drug-containing serum. LoVo cells were cultured in vitro and given different doses of rhubarb drug-containing serum culture medium for 24 h. The expression of AQP4 protein and mRNA was detected by W estern b lot and semi-quantitative RT-PCR. Results: The results showed that 4.5 g.kg-1.d-1 rhubarb serum-containing medium can inhibit the expression of AQP4 protein and mRNA in LoVo cells (P<0.01). Conclusion: The rhubarb-containing serum can inhibit the transcription and translation of AQP4 gene in LoVo cells, suggesting that the purgative effect of rhubarb may be related to the regulation of AQP4 expression.