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利用珍汕97B/密阳46所构建的RIL群体(ZM-RIL)及其相应分子遗传图谱,在海南和杭州两地试验,以稻穗下落法测得落粒率(%)为指标,进行两地数据QTL联合分析。结果表明,ZM-RIL群体的不同株系在两地间落粒率变化很大。在海南,该性状呈近似正态分布;在杭州,则呈明显偏态分布。试验共检测到7个主效应QTL,位于第1、2、3(2个)、6、7和11等6条染色体上,每个QTL影响落粒率的加性效应均不大,其幅度为1.7%~3.9%,共解释群体落粒性性状变异的8.53%。其中,有3个主效应QTL(qSH3-1、qSH3-2和qSH6)存在显著的GE互作,它们均使海南增加落粒率和杭州降低落粒率,且GE总贡献率几乎接近加性效应总贡献率,表明GE互作对落粒率具有重要影响。此外,试验还检测到5对上位性互作QTL,这些互作共解释群体落粒性性状变异的3.39%,单个互作的贡献率为0.47%~0.85%,未检测到上位性与环境的显著互作。
Using the RIL population (ZM-RIL) and its corresponding molecular genetic map constructed by Zhenshan 97B / Miryang 46, the experiment was conducted in Hainan and Hangzhou, and the drop rate (%) was measured by the drop method of rice ear as an index QTL analysis of the two data sets. The results showed that different strains of ZM-RIL population changed greatly between the two places. In Hainan, the trait is approximately normal distribution; in Hangzhou, it is obviously skewed distribution. Seven QTLs for main effects were detected in six chromosomes of chromosomes 1, 2, 3 (2), 6, 7 and 11, and the additive effects of each QTL on the drop rate were not significant. The amplitudes Was 1.7% ~ 3.9%, explaining a total of 8.53% of the variation of population dropout traits. Among them, there are three main effect QTL (qSH3-1, qSH3-2 and qSH6) there is a significant GE interaction, they both increase the rate of decline in Hainan and Hangzhou reduce the rate of falling, and the total contribution rate of GE is almost additive The total contribution rate of the effect shows that GE interaction has an important impact on the rate of falling. In addition, five pairs of epistatic QTLs were detected in the experiment. These interactions accounted for 3.39% of the variance of population dropout traits, and the contribution rate of single interaction was 0.47% -0.85%. No epistasis and environmental Significant interaction.