论文部分内容阅读
以甘蔗胚性意伤组织作原生质体培养材料，对影响甘蔗胚性意伤组织原生质体培养的因素进行了研究。结果表明，其影响因素主要有：材料的基因型；酶解前的培养基配方；原生质体培养基种类；植物生长调节剂浓度及其配比和不同培养方式等。其中，影响原生质体形成愈伤组织的最大因素是材料的基因型。５３％的参试基因型材料发生了分裂，部分形成了细胞团，其中有两个材料（一为栽培材料，一为野生资源材料）的原生质体细胞经进一步培养发育成为愈伤组织。
In order to study the factors affecting the embryogenic tissue culture of sugarcane, the embryogenic injury tissue of sugarcane was used as protoplast culture material. The results showed that the main influencing factors were: the genotype of the material; the medium formula before enzymolysis; the type of protoplast culture medium; the concentration of plant growth regulator and its proportioning and different culture methods. Among them, the impact of protoplasts callus formation is the biggest factor in the genotype of the material. Fifty-three percent of the tested genotypes split and partially formed clusters of cells. Protoplast cells with two materials (one cultivated and one wild resource) were further cultured to callus.