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采用硫酸铵沉淀，ＳｅｐｈａｄｅｘＧ－７５、ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ、ＤＥＡＥ－纤维素３２柱层析法，从肾移植病人尿中分离并纯化α＿１－微球蛋白（α＿１－ＭＧ）。用此蛋白免疫家兔、获得其抗血清。抗血清稀释度为１：１０００－１：３５００，亲和常数为３．４５×１０￣９ｌ／ｍｌ，与β＿２－ＭＧ，白蛋白、ＩｇＧ的交叉反应小于０．００３％。用氯胺Ｔ法对α＿１－ＭＧ进行碘标记，同时建立了α＿１－ＭＧ放免分析方法。经方法学鉴定，灵敏度为１．６０ｎｇ／ｍｌ，回收率均值为１１２％，批内变异系数为２．５％－３．３％，批间变异系数为２．４％－３．８％。正常人尿α＿１－ＭＧ含量为Ｘ±ＳＤ＝５．８６±４．５０μｇ／ｍｌ（ｎ＝１０２）。
Α1-microglobulin (α_1-MG) was isolated and purified from the urine of kidney transplant patients by ammonium sulfate precipitation, Sephadex G-75, ConA-Sepharose 4B, DEAE-cellulose 32 column chromatography. Rabbits were immunized with this protein to obtain their antiserum. The antiserum dilution was 1: 1000-1: 3500 and the affinity constant was 3.45 × 10 ~ 9l / ml. The cross-reaction with β_2-MG, albumin and IgG was less than 0.003%. The chloramine T method was used to label α_1-MG for iodine. At the same time, the method of radio-immunity analysis of α_1-MG was established. According to the methodological identification, the sensitivity was 1.60ng / ml, the average recovery was 112%, the coefficient of variation within the batch was 2.5% -3.3%, and the coefficient of variation between the lots was 2.4% -3.8%. Normal urinary α_1-MG content of X ± SD = 5.86 ± 4.50μg / ml (n = 102).