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目的探讨鞘氨醇-1-磷酸(S1P)对外周血单核细胞诱生的人耐受型树突状细胞(tDC)免疫学功能的调控作用。方法 RT-PCR和流式检测细胞表型以及S1P受体(S1PR s)的表达;加入S1P处理tDC,检测其对tDC表型、细胞因子表达以及信号蛋白细胞外调节蛋白激酶(ERK)磷酸化的影响。结果 tDC高表达DC标志CD209、共刺激分子CD80、CD86和成熟标志CD83;炎性细胞因子mRNA的表达低于未成熟DC,而抑炎性细胞因子、Fas-L和IDOmRNA的表达均高于iDC;tDC表达S1P的受体,S1P与受体相互作用后可引起信号蛋白ERK的磷酸化,上调tDC抑炎性细胞因子以及Fas-L mRNA的表达,对炎性细胞因子的表达无影响。结论 tDC表达S1P受体1、2、5,S1P信号活化后可上调抑炎性细胞因子mRNA的表达,而其表型和炎性细胞因子的分泌无明显变化,有利于tDC免疫耐受功能的发挥。
Objective To investigate the regulatory effect of sphingosine-1-phosphate (S1P) on the immunological function of human tolerogenic dendritic cells (tDC) induced by peripheral blood mononuclear cells. Methods RT-PCR and flow cytometry were used to detect the cell phenotype and the expression of S1P receptor (S1PR s). After adding S1P to tDC, the expression of tDC, the expression of cytokines and the phosphorylation of signal protein extracellular regulated protein kinase (ERK) Impact. Results The expression of CD209, costimulatory molecule CD80, CD86 and mature marker CD83 were higher in tDC than in imDC, while the expression of anti-inflammatory cytokines, Fas-L and IDO mRNA were higher than iDC ; tDC expression of S1P receptor, S1P interaction with the receptor can cause phosphorylation of signal protein ERK, up-regulated tDC anti-inflammatory cytokines and Fas-L mRNA expression, the expression of inflammatory cytokines had no effect. Conclusion The expression of S1P receptor 1, 2, 5 and S1P signal activated by tDC can up-regulate the expression of anti-inflammatory cytokine mRNA, while the expression of tDC and its secretion has no significant change, which is in favor of tDC immune tolerance Play