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真菌病害是世界小麦生产中的最重要的病害之一。目前,在小麦中已经鉴定出一批小麦病菌侵染诱导基因,包括病程相关基因和抗真菌水解酶基因(葡聚糖酶基因和几丁质酶基因)。最近的研究表明,植物1,3-β-葡聚糖酶参与对真菌侵染的防卫。本研究从小麦cDNA文库中克隆出一个编码小麦1,3-β-葡聚糖酶的新基因,命名为TaGlc2。该基因编码的氨基酸序列与糖苷水解酶基因家族17高度同源。采用实时定量PCR分析方法对TaGlc2基因在白粉菌侵染(Erysiphe graminis)后小麦叶片中的表达模式进行研究,发现TaGlc2基因在白粉菌侵染6h后表达明显增强,至24h达到峰值,说明该基因受白粉菌侵染诱导表达。获得了TaGlc2基因5’上游调控区序列,发现存在与病菌侵染响应有关的顺式元件。小麦TaGlc2基因在小麦白粉病抗性上可能具有重要作用。
Fungal diseases are one of the most important diseases in world wheat production. At present, a number of wheat-induced infection-inducing genes have been identified in wheat, including disease-related genes and antifungal hydrolase genes (glucanase and chitinase genes). Recent studies have shown that plant 1,3-β-glucanase is involved in the defense of fungal infections. In this study, a new gene encoding wheat 1,3-β-glucanase was cloned from a wheat cDNA library and named TaGlc2. The amino acid sequence encoded by this gene is highly homologous to glycoside hydrolase gene family17. The TaGlc2 gene expression patterns in wheat leaves after Erysiphe graminis infection were investigated by real-time quantitative PCR. The results showed that the expression of TaGlc2 gene was significantly enhanced 6 hours after infection with powdery mildew and peaked at 24h, indicating that the TaGlc2 gene Infected by powdery mildew induced expression. The sequence of the 5 ’upstream regulatory region of TaGlc2 gene was obtained, and the presence of cis elements related to the infection response of the bacteria was found. The wheat TaGlc2 gene may play an important role in wheat powdery mildew resistance.