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以前列腺特异性抗原(PSA)和前列腺特异性抗体(anti-PSA)为生物模型分子,采用电沉积技术和共价键合作用,研制了新型高灵敏电流型免疫传感器.利用具有良好导电性和热稳定性的新型有机材料[苝四甲酸二酐(PTCDA)衍生物,简写为PTC-NH2]膜具有的多孔结构,该膜可与电沉积制得的冰晶状普鲁士蓝(PB)颗粒进行层层组装镶嵌,形成多层稳定的有机-无机氧化还原复合膜以增加PB的固定量和稳定性,从而提高电极的电流响应信号;同时,通过复合膜表面丰富的氨基吸附大量纳米金以增加抗体的固定量,从而提高免疫传感器的灵敏度.利用扫描电子显微镜(SEM)和X射线光电子能谱仪(XPS)对PTC-NH2膜的形貌和结构进行表征,通过循环伏安法考察了电极修饰过程的电化学特性,详细研究了该免疫传感器的性能.该免疫电极对前列腺特异性抗原检测的线性范围为0.5～16.0ng/mL,相关系数为0.985,检测限为0.02ng/mL.实验结果表明,利用该方法制备的免疫传感器具有灵敏度高、稳定性和选择性好等优点.
Using PSA and anti-PSA as biological model molecules, a new type of high sensitive current-type immunosensor was developed by using electrodeposition and covalent bonding. The thermal stability of the novel organic material [perylenetetracarboxylic dianhydride (PTCDA) derivative, abbreviated PTC-NH2] has a porous structure that can be laminated with the electrodeposited ice-crystal Prussian blue (PB) Layer assembly inlaid to form a multi-layer stable organic-inorganic redox composite membrane to increase the fixed amount and stability of PB to improve the electrode current response signal; at the same time, a large amount of nano-gold is adsorbed by the amino groups on the surface of the composite membrane to increase the antibody , Thereby increasing the sensitivity of the immunosensor.The morphology and structure of the PTC-NH2 membrane were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), and the electrode modification was investigated by cyclic voltammetry The electrochemical properties of the immunosensor were studied in detail.The linearity of this immunosensor for detecting prostate-specific antigen was 0.5 to 16.0 ng / mL with a correlation coefficient of 0.985 Limit of 0.02ng / mL. The results show that immunization using a sensor prepared by the method has high sensitivity, good selectivity and stability.