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以福建山樱花嫩叶为试验材料,采用Trizol法、CTAB法、CTAB-Li Cl法、百泰克试剂盒法和天根试剂盒法5种方法提取福建山樱花叶片RNA,用琼脂糖凝胶电泳检测总RNA的完整性,以微量紫外分光系统检测总RNA的纯度和浓度。结果表明:Trizol法和天根试剂盒法不适于福建山樱花叶片RNA的提取;CTAB法和百泰克试剂盒法提取的RNA完整性好,纯度及浓度高,但百泰克试剂盒法提取的RNA有DNA污染;CTAB-Li Cl法提取的RNA浓度低,且含杂质。RT-PCR试验结果进一步表明CTAB法提取的RNA能够用于后续的分子生物学研究,是福建山樱花叶片RNA提取的最佳方法。
Fujian cherry saplings were used as experimental material to extract the leaves of Fujian sakura cherry by Trizol method, CTAB method, CTAB-Li Cl method, Baitai Ke kit method and day root kit method. Agarose gel electrophoresis The integrity of total RNA was assayed and the purity and concentration of total RNA was detected by micro-UV spectroscopy. The results showed that: Trizol method and day root kit method were not suitable for extraction of RNA from leaves of Fusarium saginata from Fujian province. RNA extracted by CTAB method and Baixint kit method had good integrity, high purity and high concentration. However, DNA contamination; RNA extracted by CTAB-LiCl method has low concentration and impurities. The result of RT-PCR further indicated that the RNA extracted by CTAB method can be used in subsequent molecular biology research, which is the best method for RNA extraction from leaves of F.