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目的:探讨原花青素(PC)对氨基脲(SEM)染毒小鼠睾丸组织形态及睾丸酶损伤的修复作用。方法:50只昆明种小鼠按体重随机分为5组:溶剂对照组、SEM染毒组、SEM染毒+低、中、高剂量PC保护组。SEM染毒组小鼠按56.25 mg/(kg.bw)剂量灌胃SEM溶液;低、中、高剂量PC保护组小鼠分别按100、200、400mg/(kg.bw)剂量灌胃PC溶液。除溶剂对照组灌胃等体积的去离子水外,其它每组均上午灌胃SEM、下午灌胃PC,每天1次,实验时间均为6周。实验结束后处死动物,分离双侧睾丸测定相关指标。结果:溶剂组,睾丸组织形态正常;SEM染毒组睾丸曲细精管上皮细胞层次显著减少,管壁脱落缺失,腔内未见精子等;PC保护组睾丸形态较SEM染毒组有所改善,且随PC剂量的增加改善较明显,睾丸间质有所缩小,曲细精管上皮细胞层次趋向正常等。SEM染毒组小鼠睾丸组织LDH-X、γ-GT活性降低,ACP活性升高(P<0.05);中、高剂量PC保护组LDH-X、γ-GT活性升高,ACP活性降低(P<0.05);SEM染毒组睾丸组织GSH-PX活力及GSH含量下降(P<0.05);高剂量PC保护组GSH-PX活力、GSH含量均呈增高趋势,差异均有统计学意义(P<0.05)。结论:56.25 mg/(kg.bw)剂量SEM对小鼠睾丸组织形态及其酶有损伤作用;一定剂量的PC对SEM损伤的睾丸组织形态及其酶有修复作用,其作用机制可能与抗氧化损伤有关。
Objective: To investigate the effect of proanthocyanidins (PC) on testicular histomorphology and testicular enzyme injury in semicarbazide (SEM) -induced mice. Methods: Fifty Kunming mice were randomly divided into five groups according to body weight: solvent control group, SEM exposure group, SEM exposure group + low, middle and high dose PC protection group. The mice in SEM group were treated with 56.25 mg / (kg.bw) dose of SEM solution. The mice in low, middle and high doses of PC protection group were treated with 100, 200, 400 mg / (kg.bw) . Except for the solvent control group, the same volume of deionized water was intragastrically administered, and each group was given SEM in the morning and PC in the afternoon, once a day for 6 weeks. Animals were sacrificed at the end of the experiment and indicators related to bilateral testis separation were isolated. Results: The morphology of testis in solvent group and testicle was normal. The level of testicular seminiferous tubules in SEM group was significantly decreased, the wall of the testicular tube was absent and the spermatozoa were not seen in the cavity. The morphology of testis in PC group was improved , And with the increase of PC dose, the improvement was more obvious, the interstitial of the testes reduced, and the seminiferous tubule epithelial cells tended to be normal. The activity of LDH-X, γ-GT and the activity of ACP of testis in SEM group were increased (P <0.05). The activities of LDH-X and γ-GT in medium and high- P <0.05). The activity of GSH-PX and the content of GSH in testis of rats in SEM group were significantly decreased (P <0.05). The activity of GSH-PX and the content of GSH in high-dose PC group increased with the increase of P <0.05). Conclusion: The dose of 56.25 mg / (kg · bw) SEM can damage the morphology and enzyme of testis in mice. A certain dose of PC can repair the morphology of testis and its enzyme in SEM, the mechanism may be related to anti-oxidation Damage related.